Endothelial Cells are effective models used for vascular studies. They offer an effective platform for recreating an in-vitro vascular endothelial environment and complex microvascular function. The native ability of the endothelial cells forms tabular networks, respond to biochemical and mechanical cues, and regular permeability. These cells enable molecular signalling studies related to angiogenesis, thrombosis, vascular inflammation and specific tissue microcirculation.